- Sugar chain sales service
- Sugar chain standard goods price list
- Sugar chain standard goods structural chart
- Various inquiries of ..our service.. inquiry etc. are received in the mail form.
In Glyence, a structure analysis on the asparagine-linked sugar chain (N-uniting type sugar chain) that promptly and was reliable was enabled by the abundant use of a data base and an original technology.
What is three dimension HPLC method ･･･
It is methods of analysis to which continuous analysis information by a different HPLC column of three kinds of is retrieved with database software GALAXY that has about 600 kinds of sugar chain information after the asparagine-linked sugar chain that unites with the protein is cut out with the enzyme, the candidate sugar chain is narrowed, and the structure is identified by Tomou of the standard goods candidate sugar chain and the sample sugar chain. （GALAXY : Glycoanalysis by the three axes of MS and chromatography、Sugar chain map for sugar chain structure decision and forecast）
It is recommended in such a person ･･･
- I want to examine the sugar chain contained in the serum, urine, and the organization covering it.
- I want to examine the sugar chain that changes in the generation stage (Differentiating).
- I want to examine the sugar chain that takes part in the infection of the virus and the bacillus.
- I want to examine the sugar chain that takes part in the cancer metastasis.
- I want to examine the sugar chain that takes part in an intercellular bonding.
- I want to search for the sugar chain marker who changes according to the condition.
- I want to examine the sugar chain that changes before and after the medicine processing.
- I want to know the sugar chain that unites with the protein that is refined or made to appear.
- I want to examine the sugar chain included in the protein pharmaceutical.
- I want to examine how a target sugar chain is included quantitatively.
Feature ･･･
- It has standard sugar chain and data base GALAXY that exceeds about 600 kinds, and a detailed sugar chain structure can be analyzed according to original knowhow.
- It is possible to analyze it for a scarce sugar chain like the sugar chain of making to sulfuric acid and in conspiracy the clone acid content sugar chain, etc.
- A structural isomer as which molecular mass is the same can be easily distinguished.
- A quantitative analysis can be done wholly as the serum, urine, the organization, and the cell, etc.
- An unknown sugar chain that doesn't exist in the data base can presume the sugar chain structure by the enzyme processing method.
- Not only HPLC but also the analysis that uses MALDI-TOF MS and NMR is possible.
- It is published in the thesis a lot of up to now ..the boast (trust analysis beginning in 2004).. of results of the industry-leading class.
Thesis list with name of Glyence Co.
・Glycobiology, 18(2), 145-151 (2008)
・Open Glycoscience, 1, 8-18 (2008)
・Rinsho Byori 55, 626-629 (2007)
・Glycobiology. 17(7), 713-724 (2007)
・Mol Biochem Parasitol. 147(2), 230-233 (2006)
・Biochim Biophys Acta. 1760(4), 669-677, (2006)
・Biochim Biophys Acta. 1760(4), 693-700, (2006)
・Glycobiology 15, 1051-1060 (2005)
Analytical scheme
The sample that freeze-dries or freezes is offered, and a fluorescent sign of the sugar chain (Make to Pirigelamino: make to PA) after cutting out is processed of Asparagin uniting type (N type) sugar chain by Gricoamidarze A according to two amino pyridine. The sugar chain of making to PA is separated to an acid sugar chain with which the neutral sugar chain and the Shial acid unite according to the negative ion of DEAE exchange column. Next..neutral..sugar..acidity..sugar..picture..sugar chain..element..column..with..separate. The glucose unit value is calculated from the ODS chromatogram by the glucose unit conversion macro (Shimadzu Corporation). It separates with ODS in addition by using the Amide column the separating each sugar chain picture. The glucose unit value is calculated from the Amide chromatogram as well as ODS. The obtained glucose unit value is selected and the candidate sugar chain is selected based on data base GALAXY. And, the sugar chain is identified by Tomou to the ODS column of the standard goods sugar chain and the sample sugar chain that becomes a candidate.
Step of primary analysis
- Enzyme processing：
Cutting out and gel filtration refinement of N type sugar chain by Gricoamidarze A - Fluorescent sign：
Making to PA and gel filtration refinement of sugar chain with two amino pyridine - Separation by DEAE column：
Separation of neutral sugar chain and acid sugar chain (sugar chain of making to Shiaril with which Shial acid unites) by DEAE column - Separation by ODS column：
Separation based on hydrophobe of sugar chain by ODS column and calculation of glucose unit value - Confirmation of sugar chain by MS：
Each picture that separates according to the ODS column is confirmed and it is confirmed whether it is a sugar chain molecular mass is measured by MALDI-TOF MS.
Step of the second analysis
- Separation by Amide column：
It separates based on the hydrophile of the sugar chain by the Amide column and the glucose unit value is calculated the picture after it separates by the specified ODS column. - GALAXY：
Narrowing of candidate sugar chain based on GALAXY - Sugar chain identification：
The standard goods sugar chain that becomes a candidate and the sample sugar chain are identified and the structure of the sample sugar chain is identified to the ODS column Tomou.
Unknown sugar chain(enzyme processing method and MALDI-TOF MS)
The sugar chain structure is presumed by presuming a rough kind and the number of composition sugar by MALDI-TOF MS, and analyzing the sugar chain after Ekiso Glico Shidarze is processed afterwards according to the HPLC column about an unknown sugar chain that doesn't exist in data base GALAXY. Moreover, the sugar chain structure can be presumed from the obtained glucose unit based on tool "Sugar chain tree" in data base GALAXY. These analyses become the option analyses.
Sugar chain of making to sulfuric acid analysis
The sugar chain with the sulfuric acid radical that wears the minus charge can be analyzed by three dimension HPLC method.
Glycobiology 15, 1051-1060 (2005)
Price
＜Basic service(When becoming a structural decision. )＞
| Step of primary analysis （To the enzyme processing, DEAE, the ODS column analysis, and the MALDI-TOF MS analysis） |
One analysis \500,000(excluding tax) |
| Step of the second analysis （Identification of Amide column analysis and sugar chain structure） |
One stroke \35,000(excluding tax) |
| Option analysis （Presumption of sugar chain structure by enzyme processing method） |
One stroke \50,000(excluding tax) |
＜cannot the analysis..(When not becoming a structural decision. )＞
・The gel filtration refinement goods after the enzyme is processed are done and the qualitative analysis of the sugar chain is done by the Olshinorl sulfuric acid method. \100,000 is
necessary for the amount of the sugar chain discontinuing working when it is clarified to the following work enough because of this sample check.
・\200,000 is necessary for the gel filtration refinement goods' after a fluorescent sign discontinuing working when no amount is clarified to the following work enough.
＜Example of price＞
The neutral sugar chain, the sugar chain of making to Monoshiaril (Shial acid one), the sugar chain of making to Geshiaril (the Shial acid 2), the sugar chain of making to Torishiaril (the Shial acid 3), and the sugar chain of making to Tetorashiaril (the Shial acid 4)
are separated by the DEAE column, and when 30 kinds of pictures are obtained since it separates respectively according to the ODS column, and all the structures are identified
→It becomes the first second analytical charge analytical (\500,000) + charge (\35,000×30).
Amount of necessary sample
・Sugar chain structure identification of sugar protein: Amount 100μg or more of protein when sugar chain content 30μg or more or sugar content is protein like IgG of 2〜3%
・Serum：300μl or more
・Urine：6ml or more
（Please consult about the sample quantity separately. ）
Recommended sample form
The sample cares about neither frozen goods nor the freeze-drying goods.
Delivery date
I will submit the closeout report in the following dates on the day when the sample was received.
Primary analysis ： For about two weeks
The second analysis ： About one month
Risk
When it is a sugar chain completely composed of unknown sugar even if there is an enough sample quantity, the structural estimation to say nothing of structural identification cannot be likely to be done because Ekisogricoshidarze that doesn't obtain the presumption structure because current sugar chain information cannot be used even if mass is requested with MS and corresponds doesn't exist either.
Sending method
■Method of sending sample
Please the dry ice of the amount is bundled enough when the sample is sent and send it though the sample cares about neither frozen goods nor the freeze-drying goods while frozen.
■Address
406 Ikou-renkei Incubator, Chikusa, Chikusa-ku, Nagoya,464-0858 Japan
To GLYENCE Ltd.
Tel : +81-52-745-7377 / FAX : +81-52-745-7378
Because holiday of the weekend is closing, the sample cannot be received. Please send the sample to become putting on on the weekday.
