Sugar chain structure and analysis schemes

Sugar chain profile

The fluorescent sign sample sugar chain is gradually separated with two-amino pyridine according to different HPLC column (DEAE,ODS,Amide) of three kinds of, and the elution title puts and presumes the sugar chain structure by putting and comparing the elution title of standard sugar chain. An already-known standard sugar chain reaches about 500 kinds, and is registered in original data base GALAXY(Glycoanalysis by the three axes os MS and chromatography). A structural decision of a final sample sugar chain does standard goods and the sample sugar chain of the presumed candidate sugar chain to the ODS column by doing Tomou (If it is the same structure as standard goods, become a single peak). This analysis method differs from the analysis with a mass spectroscope, and is excellent as the separation refinement method of the sugar chain. Therefore, there are a lot of merits such as "The fixed quantity analysis with reproducibility is possible" it, and "The mass spectrometry and the enzyme processing method of NMR are continuously done even if it is an unknown sugar chain that doesn't exist in the data base, and the structure can be analyzed".

The sugar chain profile changed by the condition shown in the above figure can be promptly done.